Antonio Vásquez Hidalgoantonio.vasquez@ues.edu.sv2026-01-262026-01-262026-01-23ISSN: 3107-5509https://hdl.handle.net/20.500.14492/32798The main objective was to determine penicillin-producing enzymes. Methodology. The analysis was based on the extraction of gDNA, qPCR, cDNA from Aspergillus salvadorensis that was carried out in 2024 at MACROGEN INC. by Metagenome Shotgun Sequencing Reports Illumina, the entire sequence was analyzed in 2025 to determine enzymes, proteins and secondary metabolites in their genetics. Strains of Staphylococcus aureus and Escherichia coli were inoculated in Muller Hinton dishes. Then, 0.01 microgram spores of the Aspergillus salvadorensis strain were inoculated in the center, incubated at 36 0 C x 4 days. Results. Growth of the fungus forming a halo of inhibition in the plates inoculated with gram-positive and gram-negative bacteria constitutes solid preliminary evidence of antibacterial activity associated with bioactive metabolites. MACROGEN reports by sequencing UniRef90_A2QZ81 that it has biosynthesis properties of penicillin catalyzed by the enzyme encoded acvA with a found frequency of 31.7269. The production of penicillinase makes Aspergillus salvadorensis an organism capable of interfering with antimicrobial susceptibility tests, selective media, and microbial interaction experiments. Its ability to inactivate penicillins significantly modifies the behavior of susceptible bacterial populations, allowing their survival, proliferation, and expansion the fungy under conditions that would normally inhibit them.enAttribution-NonCommercial-ShareAlike 4.0 InternationalAspergillus salvadorensisgen acvA.Trabajo de grado