Presence of enzymes and secondary metabolites clusters in DNA sequence of Aspergillus salvadorensis in the production of natural black pigments.
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Date
2025-11-03
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KR Journal of Agriculture and Biosciences-
Abstract
Objective. To determine by the Next-Generation Sequencing Illumina method to identify the genes related to the pigmentation of the fungus in the DNA sequencing of enzymes and secondary metabolites. As well as in determining the phenotypic and genotypic characteristics in general of the species A.salvadorensis. Methodology. For the identification of cluster genes in enzymes, proteins, and secondary metabolites, MACROGEN For the identification of cluster genes in enzymes, proteins, and secondary metabolites, MACROGEN used the following systems: the EggNOG system summary: Orthology Frequency within COG (Clusters of Orthologous Groups) Categories in sequencing reading. MetaCyc: Database offering detailed information on metabolic pathways, enzymes, compounds, and reactions, UniRef: The UniProt Reference Clusters (UniRef) offer clustered sets of sequences derived from the UniProt Knowledgebase, including isoforms, and selected UniParc records, EggNOG: Relative Abundance in Hierarchical Categories of COG(Clusters of Orthologous Groups) using CPM, KEGG Orthology (KO) and KEGG summary: Orthology Frequency within Main and Sub-Categories. DNA-seq. Conclusions. 14 enzymes and secondary metabolites were found in the production of black pigments produced by the fungus by oxidative stress.
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Keywords
Aspergillus salvadorensis, Macrogen, metabolites and enzymes