Immunoinformatic-Genomic Analysis of the ORF69 Protein Located in the Capsule of Aspergillus salvadorensis for the Design of Vaccines, University of El Salvador (2025-2026)
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Date
2025-04-30
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IKR Journal of Agriculture and Biosciences
Abstract
Bioinformatic scrutiny of the genome of Aspergillus salvadorensis has led to the discovery of ORF69, a sequence that stands out for its strategic potential in the fields of structural biology and fungal immunology. This open reading frame, with an extension of 281 amino acid residues, not only has a biologically coherent length for effector proteins, but also has a highly organized molecular architecture. Its topology integrates a clearly defined N-terminal signal peptide, which guarantees its translocation through the secretory pathway to the outside of the cell, positioning it as a surface protein. The relevance of this candidate is reinforced by the identification of multiple glycosylation motifs, elements that in the genus Aspergillus act as critical antigenic determinants. These residues not only stabilize the conformation of the protein in the extracellular environment, but also facilitate recognition by innate immunity receptors, such as C-type lectins, mediating direct communication with the host's immune system. The statistical robustness observed in the analysis with ORFfinder, where ORF69 is positioned as a dominant and conserved sequence, underscores its viability as a target for reverse vaccinology. This approach allows us to propose ORF69 as a central piece in the pathogen-host interaction, with the ability to induce protective immune responses of the Th1 or Th17 type. The prediction of potential antigenic epitopes of both T cells and B cells in international IEDB PROGRAMS (MHC-II binding prediction) for BepiPred/ABCpred/Emini T cell epitopes for B cell epitopes, the potential allergen with Allergome. Therefore, this finding transcends simple genomic mapping, as it establishes a scientific platform for the creation of new biotechnological tools. From the design of high-specificity diagnostic tests to the development of targeted immunotherapies, the recognition of these cell wall antigens opens a promising route to combat infections caused by this specimen, consolidating our understanding of the A. salvadorensis proteome and its clinical relevance.
Keywords: ORF69, Aspergillus salvadorensis, Type Th1, Th17, Vaccines, Epitopes.
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Keywords
ORF69, Aspergillus salvadorensis, Type Th1, Th17, Vaccines, Epitopes