Biosynthesis of penicillin encoded by gene acvA UniRef90_A2QZ81 present in circular DNA sequence of Aspergillus salvadorensis

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dc.contributor.authorAntonio Vásquez Hidalgo
dc.contributor.otherantonio.vasquez@ues.edu.sv
dc.date.accessioned2026-01-26T16:08:49Z
dc.date.available2026-01-26T16:08:49Z
dc.date.issued2026-01-23
dc.description.abstractThe main objective was to determine penicillin-producing enzymes. Methodology. The analysis was based on the extraction of gDNA, qPCR, cDNA from Aspergillus salvadorensis that was carried out in 2024 at MACROGEN INC. by Metagenome Shotgun Sequencing Reports Illumina, the entire sequence was analyzed in 2025 to determine enzymes, proteins and secondary metabolites in their genetics. Strains of Staphylococcus aureus and Escherichia coli were inoculated in Muller Hinton dishes. Then, 0.01 microgram spores of the Aspergillus salvadorensis strain were inoculated in the center, incubated at 36 0 C x 4 days. Results. Growth of the fungus forming a halo of inhibition in the plates inoculated with gram-positive and gram-negative bacteria constitutes solid preliminary evidence of antibacterial activity associated with bioactive metabolites. MACROGEN reports by sequencing UniRef90_A2QZ81 that it has biosynthesis properties of penicillin catalyzed by the enzyme encoded acvA with a found frequency of 31.7269. The production of penicillinase makes Aspergillus salvadorensis an organism capable of interfering with antimicrobial susceptibility tests, selective media, and microbial interaction experiments. Its ability to inactivate penicillins significantly modifies the behavior of susceptible bacterial populations, allowing their survival, proliferation, and expansion the fungy under conditions that would normally inhibit them.
dc.identifier.issnISSN: 3107-5509
dc.identifier.urihttps://hdl.handle.net/20.500.14492/32798
dc.language.isoen
dc.publisherIKR Journal of Agriculture and Biosciences
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectAspergillus salvadorensis
dc.subjectgen acvA.
dc.titleBiosynthesis of penicillin encoded by gene acvA UniRef90_A2QZ81 present in circular DNA sequence of Aspergillus salvadorensis
dc.typeTrabajo de grado

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